4.8 Article

Infrared Based Saliva Screening Test for COVID-19

Journal

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 60, Issue 31, Pages 17102-17107

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202104453

Keywords

COVID-19 diagnostic; Fourier transform infrared (FTIR) spectroscopy; Raman spectroscopy; saliva; SARS-CoV-2

Funding

  1. IRM beamline at Australian Synchrotron (Victoria, Australia), Australian Nuclear Science and Technology Organisation (ANSTO)
  2. Australian Government [16460, 16476]
  3. Monash Green Chemical Futures Special Project fund
  4. A2 milk foundation
  5. Jack Ma Foundation
  6. National Health and Medical Research Council of Australia (NHMRC) [1117766]
  7. Australian Research Council [CE140100011]
  8. Ramln y Cajal (RYC) Contracts Aids [RYC2019026556-I]

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The study introduces a portable infrared spectrometer for rapid detection of COVID-19 markers in saliva, demonstrating high sensitivity and specificity through modeling of experimental data. The testing method is quick, cost-effective, and suitable for mass screening, minimizing risks to healthcare workers.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented need for diagnostic testing that is critical in controlling the spread of COVID-19. We propose a portable infrared spectrometer with purpose-built transflection accessory for rapid point-of-care detection of COVID-19 markers in saliva. Initially, purified virion particles were characterized with Raman spectroscopy, synchrotron infrared (IR) and AFM-IR. A data set comprising 171 transflection infrared spectra from 29 subjects testing positive for SARS-CoV-2 by RT-qPCR and 28 testing negative, was modeled using Monte Carlo Double Cross Validation with 50 randomized test and model sets. The testing sensitivity was 93% (27/29) with a specificity of 82% (23/28) that included positive samples on the limit of detection for RT-qPCR. Herein, we demonstrate a proof-of-concept high throughput infrared COVID-19 test that is rapid, inexpensive, portable and utilizes sample self-collection thus minimizing the risk to healthcare workers and ideally suited to mass screening.

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