High-Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild-Type and B.1.1.7 SARS-CoV-2 in Unprocessed Saliva

Article Biophysics

Magnetic beads combined with carbon black -based screen-printed electrodes for COVID-19: A reliable and miniaturized electrochemical immunosensor for SARS-CoV-2 detection in saliva

Laura Fabiani et al.

Summary: The study developed an electrochemical immunoassay for rapid and smart detection of SARS-CoV-2 coronavirus in saliva, with high sensitivity, rapid analysis (30 min), miniaturization, portability, and ease of use.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Biophysics

A novel rapid detection for SARS-CoV-2 spike 1 antigens using human angiotensin converting enzyme 2 (ACE2)

Jong-Hwan Lee et al.

Summary: This study presents a novel rapid detection method for the SARS-CoV-2 S1 antigen using a matched pair of ACE2 and antibody. The detection method showed high specificity and sensitivity without cross-reactivity, making it a valuable tool in detecting the S1 antigen from COVID-19 patients.

BIOSENSORS & BIOELECTRONICS (2021)

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Letter Infectious Diseases

Evaluation of the rapid antigen test Panbio COVID-19 in saliva and nasal swabs in a population-based point-of-care study

Vanesa Agullo et al.

JOURNAL OF INFECTION (2021)

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Article Chemistry, Multidisciplinary

Detection of SARS-CoV-2 Viral Particles Using Direct, Reagent-Free Electrochemical Sensing

Hanie Yousefi et al.

Summary: The development of new methods for direct viral detection is crucial in combating the COVID-19 pandemic. This reagent-free viral sensing approach can detect the presence of viral particles and proteins within 5 minutes, providing a rapid and effective solution for identifying viruses like SARS-CoV-2.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

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Editorial Material Multidisciplinary Sciences

COVID-19 testing: One size does not fit all

Michael J. Mina et al.

SCIENCE (2021)

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Article Chemistry, Analytical

Rapid SARS-CoV-2 Detection Using Electrochemical Immunosensor

Biljana Mojsoska et al.

Summary: The development of diagnostic tests that can quickly identify infected individuals and assign treatment options is crucial during the COVID-19 pandemic. A proof-of-concept label-free electrochemical immunoassay for rapid detection of SARS-CoV-2 virus via spike surface protein has been introduced, showing high sensitivity for detection and portability for on-site diagnosis.

SENSORS (2021)

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Article Chemistry, Analytical

Rapid One-Pot Detection of SARS-CoV-2 Based on a Lateral Flow Assay in Clinical Samples

Chao Zhang et al.

Summary: This study developed a rapid detection method for SARS-CoV-2, with the entire sample-to-result workflow taking less than 40 minutes. The method achieved 100% accuracy compared to PCR-based assays, offering a potential universal platform for rapid and point-of-care detection of emerging infectious diseases.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Multidisciplinary

Neutralizing Aptamers Block S/RBD-ACE2 Interactions and Prevent Host Cell Infection

Xiaohui Liu et al.

Summary: The receptor-binding domain of the SARS-CoV-2 spike protein is crucial for mediating virus entry into human cells, making it an ideal target for blocking infection. Using aptamers specifically targeting the S/RBD, researchers were able to effectively prevent virus binding to host cells and neutralize viral particles expressing the S protein, demonstrating a promising COVID-19 therapeutic strategy.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

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Article Chemistry, Multidisciplinary

A SARS-CoV-2 Spike Binding DNA Aptamer that Inhibits Pseudovirus Infection by an RBD-Independent Mechanism**

Anton Schmitz et al.

Summary: A specific aptamer that interacts with the coronavirus SARS-CoV-2 has been identified, showing potent and specific inhibition of pseudoviral infection despite not blocking the virus's interaction with ACE2. This study opens new avenues for developing inhibitors of SARS-CoV-2 infection and highlights the potential of aptamers as drug candidates and tools for unraveling previously inaccessible infection modalities.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

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Article Chemistry, Multidisciplinary

Aptamer Blocking Strategy Inhibits SARS-CoV-2 Virus Infection

Miao Sun et al.

Summary: The research strategy focused on blocking SARS-CoV-2 infection through aptamer selection, identifying and applying the high-affinity aptamer CoV2-6, further engineered into the circular bivalent aptamer cb-CoV2-6C3 to enhance stability and inhibition efficacy.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

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Article Biophysics

The potential application of electrochemical biosensors in the COVID-19 pandemic: A perspective on the rapid diagnostics of SARS-CoV-2

Sahar Sadat Mahshid et al.

Summary: Electrochemical biosensors offer the selectivity of electrochemical signal transducers and the specificity of biomolecular recognition strategies. During the COVID-19 pandemic, electrochemical platforms have shown potential to address significant limitations of traditional diagnostic platforms, making them strong candidates for rapid detection of SARS-CoV-2 infection.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Biophysics

Hitting the diagnostic sweet spot: Point-of-care SARS-CoV-2 salivary antigen testing with an off-the-shelf glucometer

Naveen K. Singh et al.

Summary: The study introduces an aptamer-based SARS-CoV-2 salivary antigen assay that utilizes low-cost reagents and an off-the-shelf glucometer. This test is highly scalable, provides rapid results within 1 hour, and can accurately detect viral loads in infected specimens while distinguishing them from uninfected controls.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Medicine, General & Internal

Rapid antigen screening of asymptomatic people as a public health tool to combat COVID-19

Kevin L. Schwartz et al.

CANADIAN MEDICAL ASSOCIATION JOURNAL (2021)

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Article Biotechnology & Applied Microbiology

Selection and characterization of structure-switching DNA aptamers for the salivary peptide histatin 3

Yagya R. Ojha et al.

Summary: This study reported single-stranded DNA aptamers that undergo structural conformational changes upon binding to salivary peptide histatin 3 for the first time. Through the SELEX process, four unique aptamer candidates with binding affinity and specificity to histatin 3 were identified. The best performing candidate exhibited an equilibrium dissociation constant (K-d) value of 1.97 +/- 0.48 mu M, showing potential for refined sensing assays for the detection and quantification of histatin 3 in biological media.

JOURNAL OF BIOTECHNOLOGY (2021)

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Article Infectious Diseases

Panbio™ rapid antigen test for SARS-CoV-2 has acceptable accuracy in symptomatic patients in primary health care

Oana Bulilete et al.

Summary: The study evaluated the accuracy of Panbio(TM) Ag-RDT in symptomatic patients and found it to perform relatively well in those presenting within five days of symptom onset. However, the data do not support the sole use of Panbio(TM) Ag-RDT in asymptomatic individuals.

JOURNAL OF INFECTION (2021)

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Article Multidisciplinary Sciences

Rapid electrochemical detection of coronavirus SARS-CoV-2

Thanyarat Chaibun et al.

Summary: The researchers developed an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification for rapid detection of SARS-CoV-2, capable of detecting as low as 1 copy/mu L of N and S genes in clinical samples. The sensor showed a 100% concordance with qRT-PCR results in clinical sample evaluation.

NATURE COMMUNICATIONS (2021)

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Article Chemistry, Multidisciplinary

Rapid, Ultrasensitive, and Highly Specific Diagnosis of COVID-19 by CRISPR-Based Detection

Xiong Zhu et al.

Summary: The study developed a novel diagnostic test for COVID-19, called COVID-19 MCCD, by combining MCDA with CRISPR-Cas12a technology, which is sensitive, rapid, and reliable for the diagnosis of SARS-CoV-2 infection.

ACS SENSORS (2021)

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Article Infectious Diseases

Clinical performance of the Abbott Panbio with nasopharyngeal, throat, and saliva swabs among symptomatic individuals with COVID-19

William Stokes et al.

Summary: The Panbio antigen test helps combat COVID-19 by reliably detecting SARS-CoV-2 in symptomatic adults within 7 days of symptom onset using nasopharyngeal swabs. However, throat and saliva swabs are not suitable for Panbio testing.

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES (2021)

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Article Virology

Evaluation of three rapid lateral flow antigen detection tests for the diagnosis of SARS-CoV-2 infection

A. E. Jaaskelainen et al.

Summary: The study evaluated the performance of three rapid antigen detection tests, showing high specificity, sensitivity, and agreement. RADTs were found to be less sensitive than RT-PCR, but they offer advantages in terms of testing speed, convenience, and cost compared to RT-PCR. Repeated testing in appropriate settings may improve overall performance.

JOURNAL OF CLINICAL VIROLOGY (2021)

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Article Chemistry, Multidisciplinary

Rapid One-Step Detection of Viral Particles Using an Aptamer-Based Thermophoretic Assay

Jinqi Deng et al.

Summary: The study introduces a one-step thermophoretic assay for rapid and sensitive detection of viral particles, specifically targeting the spike protein of SARS-CoV-2. The assay achieved a low limit of detection in a short time without the need for pretreatment, demonstrating high accuracy when detecting synthetic samples. This thermophoretic assay could potentially enhance diagnostic tools for viral pathogens due to its simplicity, speed, and cost-effectiveness.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

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Article Immunology

A Saliva-Based RNA Extraction-Free Workflow Integrated With Cas13a for SARS-CoV-2 Detection

Iqbal Azmi et al.

Summary: A major bottleneck in scaling-up COVID-19 testing is the need for sophisticated instruments and trained healthcare professionals, as well as constraints in the supply chain. CASSPIT introduces a new approach that allows direct use of saliva samples for detection without the extra RNA extraction step.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2021)

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Letter Medicine, General & Internal

Neutralising antibody activity against SARS-CoV-2 VOCs B.1.617.2 and B.1.351 by BNT162b2 vaccination

Emma C. Wall et al.

LANCET (2021)

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Article Biochemistry & Molecular Biology

Diverse high-affinity DNA aptamers for wild-type and B.1.1.7 SARS-CoV-2 spike proteins from a pre-structured DNA library

Jiuxing Li et al.

Summary: In vitro selection experiments identified DNA aptamers, MSA1 and MSA5, with strong binding affinity to the S1 subunit of the SARS-CoV-2 spike protein. These aptamers demonstrated comparable affinities for various forms of S1 protein and also recognized the pseudotyped lentivirus of SARS-CoV-2, highlighting their potential as diagnostic tools for COVID-19 detection. The development of a colorimetric sandwich assay using MSA1 further confirmed the utility of these aptamers in detecting the virus in saliva samples.

NUCLEIC ACIDS RESEARCH (2021)

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Article Nanoscience & Nanotechnology

Rationally Designed Multivalent Aptamers Targeting Cell Surface for Biomedical Applications

Meihua Lin et al.

Summary: Specific interactions between ligands and receptors on cell surface are crucial in cell biology. Nucleic acid aptamers, as common ligands, enable specific recognition and tight binding to membrane protein receptors for potential cancer diagnosis and targeted therapy. However, challenges exist due to rapid degradation and clearance, prompting the development of multivalent aptamers for enhanced performance in physiological conditions.

ACS APPLIED MATERIALS & INTERFACES (2021)

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Article Chemistry, Analytical

Detection of the SARS-CoV-2 spike protein in saliva with Shrinky-Dink© electrodes

Julia A. Zakashansky et al.

Summary: By utilizing low-cost electrodes and aptamer technology, we can detect the spike protein of SARS-CoV-2 in saliva with a sensitivity down to 1 ag/mL, making it suitable for large-scale viral infection detection.

ANALYTICAL METHODS (2021)

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Article Medicine, General & Internal

Real-life validation of the Panbio™ COVID-19 antigen rapid test (Abbott) in community-dwelling subjects with symptoms of potential SARS-CoV-2 infection

Hendrik Gremmels et al.

Summary: The antigen rapid test showed high specificity and sensitivity in community-dwelling residents in the Netherlands and Aruba, especially when using a Ct value <32 cycles as the cut-off for RT-qPCR positivity. This test could play a significant role in controlling viral spread.

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Potent mouse monoclonal antibodies that block SARS-CoV-2 infection

Youjia Guo et al.

Summary: In response to the global pandemic of COVID-19, researchers have isolated various human recombinant monoclonal antibodies that can neutralize SARS-CoV-2 infection. However, there is still a need for suitable monoclonal antibodies for molecular pathology research. By producing mouse anti-SARS-CoV-2 spike monoclonal antibodies, researchers have created a useful tool for future research and potential application in clinical settings.

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Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor

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Human monoclonal antibodies block the binding of SARS-CoV-2 spike protein to angiotensin converting enzyme 2 receptor

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Potent binding of 2019 novel coronavirus spike protein by a SARS coronavirus-specific human monoclonal antibody

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Performance of Abbott ID Now COVID-19 Rapid Nucleic Acid Amplification Test Using Nasopharyngeal Swabs Transported in Viral Transport Media and Dry Nasal Swabs in a New York City Academic Institution

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Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays

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High-Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild-Type and B.1.1.7 SARS-CoV-2 in Unprocessed Saliva

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