4.8 Article

Neutrophil-Selective Fluorescent Probe Development through Metabolism-Oriented Live-Cell Distinction

Journal

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 60, Issue 44, Pages 23743-23749

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202108536

Keywords

cell recognition; fatty acids; fluorescent probes; metabolism

Funding

  1. Institute for Basic Science (IBS) [IBS-R007-A1]
  2. POSTECH (Pohang University of Science and Technology)

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This study presented the first fluorescent probe NeutropG for specific distinction and imaging of active neutrophils, with a selective staining mechanism elucidated as metabolism-oriented live-cell distinction (MOLD). NeutropG was successfully applied to accurately quantify neutrophil levels in fresh blood samples, showing a high correlation with the current clinical method.
Human neutrophils are the most abundant leukocytes and have been considered as the first line of defence in the innate immune system. Selective imaging of live neutrophils will facilitate the in situ study of neutrophils in infection or inflammation events as well as clinical diagnosis. However, small-molecule-based probes for the discrimination of live neutrophils among different granulocytes in human blood have yet to be reported. Herein, we report the first fluorescent probe NeutropG for the specific distinction and imaging of active neutrophils. The selective staining mechanism of NeutropG is elucidated as metabolism-oriented live-cell distinction (MOLD) through lipid droplet biogenesis with the help of ACSL and DGAT. Finally, NeutropG is applied to accurately quantify neutrophil levels in fresh blood samples by showing a high correlation with the current clinical method.

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