Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 60, Issue 36, Pages 19957-19964Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202107192
Keywords
anaerobic sulfatase maturase; gem-diol; oxidation; posttranslational modification; RiPP
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Funding
- National Key Research and Development Program [2016 Y F A0501302, 2018Y F A0900402]
- National Natural Science Foundation of China [21822703, 21921003, 32070050]
- Innovative research team of high-level local universities in Shanghai
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The study reveals a enzyme named three-residue cyclophane forming enzyme (3-CyFE) exhibiting remarkable catalytic promiscuity, producing cyclophane and aminomalonate. Bioinformatic analysis indicates that 3-CyFEs have evolved from a common ancestor with anaerobic sulfatase maturases (anSMEs) and share a similar set of catalytic residues.
Aminomalonate (Ama) is a widespread structural motif in Nature, whereas its biosynthetic route is only partially understood. In this study, we show that a radical S-adenosylmethionine (rSAM) enzyme involved in cyclophane biosynthesis exhibits remarkable catalytic promiscuity. This enzyme, named three-residue cyclophane forming enzyme (3-CyFE), mainly produces cyclophane in vivo, whereas it produces formylglycine (FGly) as a major product and barely produce cyclophane in vitro. Importantly, the enzyme can further oxidize FGly to produce Ama. Bioinformatic study revealed that 3-CyFEs have evolved from a common ancestor with anaerobic sulfatase maturases (anSMEs), and possess a similar set of catalytic residues with anSMEs. Remarkably, the enzyme does not need leader peptide for activity and is fully active on a truncated peptide containing only 5 amino acids of the core sequence. Our work discloses the first ribosomal path towards Ama formation, providing a possible hint for the rich occurrence of Ama in Nature.
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