Biochemistry-Guided Prediction of the Absolute Configuration of Fungal Reduced Polyketides

Highly reducing polyketide synthases (HR-PKSs) produce structurally diverse polyketides (PKs). The PK diversity is constructed by a variety of factors, including the beta-keto processing, chain length, methylation pattern, and relative and absolute configurations of the substituents. We examined the stereochemical course of the PK processing for the synthesis of polyhydroxy PKs such as phialotides, phomenoic acid, and ACR-toxin. Heterologous expression of a HR-PKS gene, a trans-acting enoylreductase gene, and a truncated non-ribosomal peptide synthetase gene resulted in the formation of a linear PK with multiple stereogenic centers. The absolute configurations of the stereogenic centers were determined by chemical degradation followed by comparison of the degradation products with synthetic standards. A stereochemical rule was proposed to explain the absolute configurations of other reduced PKs and highlights an error in the absolute configurations of a reported structure. The present work demonstrates that focused functional analysis of functionally related HR-PKSs leads to a better understanding of the stereochemical course.

Automated summary

Highly reducing polyketide synthases (HR-PKSs) are responsible for the production of structurally diverse polyketides (PKs) through a complex stereochemical process. By examining the synthesis of polyhydroxy PKs, researchers were able to determine the absolute configurations of stereogenic centers using chemical degradation and comparison with synthetic standards. This study proposes a stereochemical rule to explain the absolute configurations of reduced PKs and highlights discrepancies in previously reported structures.