Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 60, Issue 44, Pages 23545-23551Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202108124
Keywords
image processing; interface structure; lanthanide nanomaterials; lifetime multiplexing; NIR-II fluorescence
Categories
Funding
- National Key R&D Program of China [2017YFA0207303]
- National Natural Science Foundation of China [NSFC 22088101, 21725502, 51961145403, 21904023, 2210040480]
- Research Program of Science and Technology Commission of Shanghai Municipality [20490710600, 20YF1402200, 20JC1411700, 19490713100, 20S31903700]
- King Saud University [RG-1435-002]
Ask authors/readers for more resources
A series of Er3+ doped double interface fluorescent nanoprobes (Er-DINPs) with strong fluorescence intensity and easily distinguishable fluorescence lifetime were developed to enable high-fidelity lifetime multiplexed imaging. Both in vitro and in vivo experimental results confirmed the advantage of these probes for high-fidelity multiplexed lifetime bioimaging.
Fluorescence lifetime imaging provides more possibility of in vivo multiplexing in second near infrared (NIR-II) window. However, it still faces the obstacle that fluorescent probes with differentiable lifetime often exhibit quite different fluorescence intensity, especially the short lifetime usually accompanies with a weak fluorescence intensity, resulting in the difficulty for simultaneously decoding multiplexed lifetime information due to the interference of background noise. To facilitate high-fidelity lifetime multiplexed imaging, we developed a series of Er3+ doped double interface fluorescent nanoprobes (Er-DINPs): alpha-NaYF4@NaErF4: Ce@NaYbF4@NaErF4: Ce@NaYF4 with strong fluorescence intensity and easily distinguishable fluorescence lifetime. Both in vitro and in vivo experimental results confirmed the advantage of these probes with comparable fluorescence intensity for high-fidelity multiplexed lifetime bioimaging.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available