Journal
ANDROLOGIA
Volume 53, Issue 9, Pages -Publisher
WILEY
DOI: 10.1111/and.14167
Keywords
cryopreservation; human spermatozoon; motility; oxidative stress; Quercetin
Categories
Funding
- Arak University
Ask authors/readers for more resources
The study showed that Quercetin supplementation can improve the quality of cryopreserved human semen by reducing MDA levels and DNA damage, and increasing total motility, progressive motility, normal morphology, viability, and antioxidant enzyme activity.
The study has aimed to investigate the effect of Quercetin, as a potent antioxidant, on preventing the negative effects of freezing process on sperm quality of patients with Asthenospermia. Semen sample from 25 patients was randomly divided into three groups; fresh, control and Quercetin (50 mu M). Seven days after freezing, samples were thawed at ambient temperature. Total motility, progressive sperm motility, normal morphology, viability and DNA integrity were evaluated according to WHO criteria, Papanicolaou, eosin- nigrosine and acridine orange staining respectively. In addition, the health of sperm membrane and mitochondrial membrane potential was assessed by HOS test and rhodamine staining. MDA and antioxidant enzyme activity were also evaluated using ELISA method. In contrast to the fresh group, the mean level of MDA and DNA damage had significant increase in the control group and decreased significantly sperm quality (p <= 0.001). The mean percentage of total motility and progressive motility, normal morphology, viability and antioxidant enzyme activity had significant increase in the Quercetin group than the control group. In the Quercetin group, the MDA level and DNA damage also had significant reduction in comparison with the control group (p <= 0.001). Therefore, the Quercetin supplementation improves the quality of cryopreserved human semen.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available