Journal
ANALYTICAL SCIENCES
Volume 37, Issue 6, Pages 897-903Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.20P219
Keywords
Nanoelectrospray ionization source; 3D printing; monolithic spray emitter; microfluidic hydrodynamic focusing; Chip-MS platform
Categories
Funding
- program of introducing talents of discipline to universities of China (111 Program) [B17019]
- self-determined research funds of CCNU from the colleges' basic research and operation of MOE [CCNU19KYZHSY07, CCNU19TS053]
Ask authors/readers for more resources
The study introduces a 3D printing method to develop a microfluidic nanoelectrospray ionization source (NIS), which is compatible with various nanoflows and shows excellent performance in detecting organic compounds, insulin, and lysozyme.
Nanoelectrospray ionization (nESI) mass spectrometry (MS) is an ideal detection method for microfluidic chips, and its performances depend on nESI emitters. However, the fabrication of monolithic nESI emitters in chips was difficult. Herein, we propose a three-dimensional (3D) printing method to develop a microfluidic nanoelectrospray ionization source (NIS), composed of a nESI emitter and other components. Firstly, the NIS was compatible with a 50 - 500 nL min(-1) nanoflows by imposing 3D hydrodynamic focusing to compensate for the total flow rate, achieving a 7.2% best relative standard deviation in the total ion current (TIC) profiles. Additionally, it was applied to probe thirteen organic chemicals, insulin, and lysozyme with adequate signal-to-noise ratios and an accuracy of m/z between 9.02 x 10(-1) and 1.48 x 10(3) ppm. Finally, the NIS achieved comparable limits of detection compared with its commercial counterpart. Considering the standardized preparation of NIS, it would be a potential option to develop 3D-printed customized Chip-MS platforms.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available