Journal
ANALYTICAL CHEMISTRY
Volume 93, Issue 25, Pages 8978-8985Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c01568
Keywords
-
Categories
Funding
- NSFC [21672083, 21877048, 22077048]
- Guangxi University [A3040051003]
Ask authors/readers for more resources
Photoacoustic imaging, combining the advantages of optical and ultrasound imaging, is a promising tool for monitoring the steady-state changes of carbon monoxide (CO) in living organisms. A newly developed activatable PA probe, MTR-CO, showed high sensitivity, specificity, and biocompatibility for CO detection in vivo, providing a potential strategy for disease monitoring.
Photoacoustic (PA) imaging is an emerging biomedical imaging modality that combines the advantages of optical and ultrasound imaging. Carbon monoxide (CO), which is a vital endogenous cell-signaling molecule in the human body, exerts critical physiological functions such as anti-inflammatory, antiapoptotic, and antiproliferative. The imbalance of CO homeostasis is also associated with numerous diseases. Therefore, it is critically important to noninvasively monitor the steady-state changes of CO in vivo. However, the activatable photoacoustic (PA) probes for detecting CO-associated complicated diseases have not yet developed. In this work, we developed the first turn-on PA probe (MTR-CO) to visualize the CO level in the lipopolysaccharide (LPS)-induced acute inflammation murine model through PA imaging technology. MTR-CO is composed of a near-infrared absorption cyanine-like dye (MTR-OH) and allyl formate, showing a 10.2-fold PA signal enhancement at 690 nm upon activation by CO. Furthermore, the results revealed that MTR-CO has high sensitivity, excellent specificity, and good biocompatibility for CO in vivo. MTR-CO was then applied for PA imaging of CO in cells and for monitoring the development of acute inflammation in the murine model by tracking the changes of the CO level. These findings provide a promising strategy for accurately detecting the steady-state changes of CO in living organisms.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available