4.8 Article

Highly Sensitive Colorimetric/Surface-Enhanced Raman Spectroscopy Immunoassay Relying on a Metallic Core-Shell Au/Au Nanostar with Clenbuterol as a Target Analyte

Journal

ANALYTICAL CHEMISTRY
Volume 93, Issue 23, Pages 8362-8369

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c01487

Keywords

-

Funding

  1. National Natural Science Foundation of China [31972150, 21675127]
  2. key R&D Projects in Shaanxi Province [2019NY-109]
  3. Key Industries Innovation Chain Project of Shaanxi Province [2019ZDLSF07-08]
  4. Shaanxi Provincial Science Fund for Distinguished Young Scholars [2018JC-011]
  5. Natural Science Foundation Project in Guangdong Provincial [2020A1515010778]
  6. 417 Development Project of Qinghai Provincial Key Laboratory [2017-ZJ-Y10]

Ask authors/readers for more resources

A dual-model colorimetric/SERS lateral flow immunoassay based on a metallic core-shell Au/Au nanostar was constructed for ultrasensitive determination of clenbuterol. The method achieved visual detection of clenbuterol at 5 ng/mL and quantitative detection as low as 0.05 ng/mL, significantly lower than traditional AuNPs-based immunoassays.
Lateral flow immunoassay (LFIA) has emerged as an effective technique in the field of food safety and environmental monitoring. However, sensitive and quantitative detection is still challenging for LFIAs in complex environments. In this work, a dual-model colorimetric/SERS lateral flow immunoassay for ultrasensitive determination of clenbuterol was constructed based on a metallic core-shell Au/Au nanostar acting as a multifunction tag. Raman reporter molecules are located between the core (AuNP) and shell (Au nanostar) to form a sandwich structure, which contributes to eliminate the environmental interference and improve the detection stability. In addition, the Au/Au nanostar provides a much higher Raman enhancement due to the presence of sharp tips and larger surface roughness in comparison with gold nanoparticles (AuNPs). Thus, on the basis of the antibody-antigen interaction, the dual-model immunoassay can produce strong colorimetric and surface-enhanced Raman spectroscopy (SERS) signals for highly sensitive detection of the target analyte, clenbuterol. Under optimal conditions, clenbuterol could be detected by the colorimetric model with a visual detection limit of 5 ng/mL. Meanwhile, the SERS signal of the Au/Au nanostar was accumulated on the test line for the SERS model detection with a quantitative detection limit as low as 0.05 ng/mL, which is at least 200-fold lower than that of the traditional AuNPs-based immunoassay. Furthermore, recovery rates of the proposed method in food samples were 86-110%. This dual-model immunoassay provides an effective tool for antibiotic residues analysis and demonstrates a broad potential for future applications in food safety monitoring.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available