Journal
ANALYTICAL CHEMISTRY
Volume 93, Issue 29, Pages 10167-10174Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c01163
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Funding
- National Natural Science Foundation of China [32001792, 32022069]
- Open Project Program of the Beijing Laboratory of Food Quality and Safety, Beijing Technology and Business University [FQS-202009]
- Newton International Fellowships Programme of Royal Society [NIF/R1/192293]
- Special Scientific Research Project of Education Department of Shaanxi Provincial Government [20JK0529]
- PhD Start-up Capital of Shaanxi University of Science Technology [2019BJ-16]
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Surface-enhanced Raman scattering (SERS) is a powerful tool for biosensors due to its ultrahigh sensitivity and unique fingerprint information. This study proposed a sensitive on-site nucleic acid detection strategy based on CRISPR/Cas12a with trans-cleavage activity on ssDNA linkers utilized to capture liposomes, enabling dual-mode detection of target DNA with SERS and naked eye.
Surface-enhanced Raman scattering (SERS) has been recognized as a powerful tool for biosensors due to the ultrahigh sensitivity and unique fingerprint information. However, there are some limitations in trace target nucleic acid detection for the restricted signal- transducing and amplification strategies. Inspired by CRISPR/Cas12a with specific target DNA-activated collateral single-strand DNA (ssDNA) cleavage activity and liposome with signal molecule-loading properties, we first proposed a sensitive SERS-based on-site nucleic acid detection strategy mediated by CRISPR/Cas12a with trans-cleavage activity on ssDNA linkers utilized to capture liposomes. Liposomes loading two kinds of signal molecules, 4-nitrothiophenol (4-NTP) and cysteine, could achieve the dual-mode detection of target DNA with SERS and naked eye, respectively. The promptly amplified signals were initiated by the triggered breakdown of signal molecule-loaded liposomes. Emancipated 4-NTP, a biological-silent Raman reporter, would achieve highly selective and sensitive SERS measurement. Released cysteine induced the aggregation of plasmonic gold nanoparticles, leading to an obvious red to blue colorimetric shift to realize portable naked-eye detection. With this strategy, target nucleic acid concentration was dexterously converted into SERS and visualization signals and could be detected as low as 100 aM and 10 pM, respectively. The approach was also successfully applied to determine meat adulteration, achieving the detection of a low adulteration ratio in the complicated food matrix. We anticipate that this strategy will not only be regarded as a universal platform for the on-site detection of food authenticity but also broaden SERS application for the accurate determination of diverse biomarkers.
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