Mitochondria-Targeting and Reversible Near-Infrared Emissive Iridium(III) Probe for in vivo ONOO-/GSH Redox Cycles Monitoring

Peroxynitrite (ONOO-) and glutathione (GSH), two unique reactive species, play an essential regulating role in the oxidation and antioxidation in the living body and are closely associated with various physiological and pathological processes, like cancer, cardiovascular disorders, diabetes, inflammation, Alzheimer's disease, and hepatotoxicity. Thus, it is crucial to study mitochondria ONOO-/GSH redox cycles by an effective molecular tool. In this work, a mitochondria-targeting and redox-reversible near-infrared (NIR) phosphorescent iridium complex, Ir-diol, has been synthesized and used for the detection and imaging of a cellular redox state by visualizing endogenous ONOO-/GSH content. Ir-diol shows excellent photophysical properties, including NIR emission (the maximum emissive wavelength for 704 nm, approximately) and high phosphorescent quantum yield (Phi = 0.136) and exhibits high sensitivity and selectivity toward ONOO-/GSH redox cycles in aqueous solution and living cells. Therefore, these features, combined with low cytotoxicity and excellent cell permeability, enable probe Irdiol to monitor the changes of the intracellular ONOO-/GSH level induced by drug both in vitro and in vivo.

Automated summary

Peroxynitrite and glutathione play essential roles in regulating oxidation and antioxidation in the body, and studying mitochondria ONOO-/GSH redox cycles is crucial for understanding various diseases. The synthesized Ir-diol fluorescent probe exhibits excellent properties, showing high sensitivity and selectivity towards ONOO-/GSH in aqueous solution and living cells.