Journal
ANALYTICAL CHEMISTRY
Volume 93, Issue 36, Pages 12391-12399Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c02305
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Funding
- University of Duisburg-Essen, Centre for Nanointegration Duisburg-Essen (CENIDE)
- University Hospital Essen
- COVID-19 Forschungsnetzwerk Universi-tatsmedizin
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The study compared the performance of a conventional lateral flow assay (LFA) with a surface-enhanced Raman scattering (SERS)-based LFA test in detecting SARS-CoV-2-specific IgM/IgG in sera of COVID-19 patients. The custom-built SERS reader showed at least an order of magnitude higher sensitivity than conventional LFAs with naked-eye detection. SERS detection in a purified system showed a sensitivity that is 7 orders of magnitude higher than naked-eye detection, indicating the high potential of SERS-based LFAs in point-of-care testing.
As an immune response to COVID-19 infection, patients develop SARS-CoV-2-specific IgM/IgG antibodies. Here, we compare the performance of a conventional lateral flow assay (LFA) with a surface-enhanced Raman scattering (SERS)-based LFA test for the detection of SARS-CoV-2-specific IgM/IgG in sera of COVID-19 patients. Sensitive detection of IgM might enable early serological diagnosis of acute infections. Rapid detection in serum using a custom-built SERS reader is at least an order of magnitude more sensitive than the conventional LFAs with naked-eye detection. For absolute quantification and the determination of the limit of detection (LOD), a set of reference measurements using purified (total) IgM in buffer was performed. In this purified system, the sensitivity of SERS detection is even 7 orders of magnitude higher: the LOD for SERS was ca. 100 mu g/mL compared to ca. 1 mu g/mL for the naked-eye detection. This outlines the high potential of SERS-based LFAs in point-of-care testing once the interference of serum components with the gold conjugates and the nitrocellulose membrane is minimized.
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