Journal
ANALYTICAL BIOCHEMISTRY
Volume 629, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2021.114294
Keywords
Monoamine oxidase; MAO activity; DNPH spectrophotometry
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The study aimed to modify a spectrophotometric assay for MAO activity evaluation with 2,4-dinitrophenylhydrazine, allowing accurate measurement of specific enzyme activity using different substrates.
The aim of the study was to modify a simple and widely used spectrophotometric assay for MAO activity evaluation with 2,4-dinitrophenylhydrazine. A modified procedure includes molar absorption coefficients of 2,4DNP-hydrazone benzaldehyde and 2,4-DNP-hydrazone 5-hydroxyindolylacetaldehyde as 2.3 x 104mol- 1l cm-1 and 1.0 x 104 mol- 1l cm-1, respectively. Such an approach allows to express specific enzyme activity as nmol product formed/min/mg protein.
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