Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 413, Issue 24, Pages 6191-6198Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-021-03410-8
Keywords
Molecularly imprinted polymers; Graphene oxide; Composites; Quartz crystal microbalance; Dengue type 1 virus
Funding
- University of Vienna
- Department of Physical Chemistry, Faculty for Chemistry, University of Vienna, Austria
- Faculty of Science, Kasetsart University, Thailand
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Graphene oxide-molecularly imprinted polymer composites (GO-MIP) have been developed as recognition materials for sensing due to their excellent properties. A new sensor material blending GO with MIP was designed to detect the harmful dengue type 1 virus (DENV-1), showing improved sensitivity and selectivity. The composite sensor exhibited a linear response to DENV-1 in the concentration range from 10(0) to 10(3) pfu mL(-1), with limits of detection and quantification of 0.58 and 1.94 pfu mL(-1), respectively.
Graphene oxide-molecularly imprinted polymer composites (GO-MIP) have attracted significant attention as recognition materials in sensing due to their outstanding properties in terms of electrical and thermal conductivity, high mechanical modulus, and the comparably straightforward way to functionalize them. The aim of this study was to design a MIP-based sensor recognition material and enhance its sensitivity by blending it with GO for sensing a harmful dengue hemorrhagic fever pathogen, namely the dengue type 1 virus (DENV-1). Polymer composites comprising GO incorporated to an acrylamide (AAM)/methacrylic acid (MAA)/methyl methacrylate (MMA)/N-vinylpyrrolidone (VP) copolymer were synthesized and compared to the pure MIP, i.e., the copolymer without GO. The pure polymer revealed a zeta potential of + 9.9 +/- 0.5 mV, whereas GO sheets prepared have a zeta potential of - 60.3 +/- 2.7 mV. This results in an overall zeta potential of - 11.2 +/- 0.2 mV of the composite. Such polymer composites seem appropriate to bind the positively charged DENV-1 particle (+ 42.2 +/- 2.8 mV). GO-MIP coated onto 10-MHz quartz crystal microbalance (QCM) sensors indeed revealed two times sensitivity compared to sensors based on the pure MIP. Furthermore, GO-polymer composites revealed imprinting factors of up to 21, compared to 3 of the pure MIP. When plotting the sensor characteristic in a semilogarithmic way, the composite sensor shows the linear response to DENV-1 in the concentration range from 10(0) to 10(3) pfu mL(-1). The corresponding limits of detection (S/N = 3) and quantification (S/N = 10) are 0.58 and 1.94 pfu mL(-1), respectively. Furthermore, imprinted polymer composites selectively bind DENV-1 without significant interference: DENV-2, DENV-3, DENV-4, respectively, yield 13-16% of DENV-1 signal. The sensor requires only about 15-20 min to obtain a result.
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