Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 413, Issue 25, Pages 6267-6277Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-021-03581-4
Keywords
Camphor; Fluorescence probe; Fe2+; Biological imaging
Funding
- National Natural Science Foundation of China [32071707]
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A novel fluorescent probe CBO was synthesized for detecting Fe2+ with high sensitivity, showing excellent performance in fluorescence imaging of Fe2+ in organisms.
In this work, a novel fluorescent probe CBO was synthesized for detecting Fe2+ using the natural monoterpenketone camphor as the starting material. The probe CBO displayed turn-on fluorescence to Fe2+ accompanied by the solution change from colorless to green. As expected, there was an excellent linear relationship between the fluorescence intensity of probe CBO and the concentration of Fe2+ (0-20 mu M), and the detection limit was as low as 1.56x10(-8) M. In particular, CBO could selectively sense Fe2+ more than other analytes (Fe3+ included) through the N-oxide strategy, and quickly responded to Fe2+ (60 s) over a wide pH (4-14) range. Additionally, based on the rapid fluorescence response of CBO to Fe2+, a simple test strip-based detector was designed for boosting practical applicability. The probe CBO had been successfully applied to the fluorescence imaging of Fe2+ in onion cells and living zebrafish. The probe CBO was a powerful tool of detecting Fe2+ level in organisms, which was of significance to understand the role of Fe2+ in Fe2+-related physical processes and diseases.
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