Development of carbon dot-thiochrome-based sensing system for ratiometric fluorescence detection of D-penicillamine

A simple and rapid ratiometric fluorescent sensing system for d-penicillamine (D-PA) determination is developed based on yellow carbon dots (Y-CDs) combined with thiochrome (oxVB(1)) for the first time. The oxidization of thiamine (VB1) can be catalyzed by Alkaline-hydrolyzed artemisinin (a-ART) to form oxVB(1), which leads to the occurrence of fluorescence emission peak at 466 nm. Furthermore, the oxidation reaction between a-ART and VB1 could be inhibited by D-PA, and accompanied with the decrease of fluorescence at 466 nm. However, the fluorescence peak of Y-CDs as an internal reference at 566 nm was almost unchanged. The ratiometric signal changes contributed to a robust and sensitive D-PA sensing. Under the optimal condition, a good linear response for the D-PA detection was obtained in the ranges of 0.5-50 mu M with a detection limit of 0.33 mu M. In addition, Y-CDs and thiochrome-based sensing system was applied to D-PA determination in real samples and obtained acceptable results.

Automated summary

A simple and rapid ratiometric fluorescent sensing system for determining D-penicillamine has been developed using yellow carbon dots and thiochrome combination. The system showed a good linear response and acceptable results in real sample testing.