Antibody- and nucleic acid-based lateral flow immunoassay for Listeria monocytogenes detection

Listeria monocytogenes is an invasive opportunistic foodborne pathogen and its routine surveillance is critical for protecting the food supply and public health. The traditional detection methods are time-consuming and require trained personnel. Lateral flow immunoassay (LFIA), on the other hand, is an easy-to-perform, rapid point-of-care test and has been widely used as an inexpensive surveillance tool. In recent times, nucleic acid-based lateral flow immunoassays (NALFIA) are also developed to improve sensitivity and specificity. A significant improvement in lateral flow-based assays has been reported in recent years, especially the ligands (antibodies, nucleic acids, aptamers, bacteriophage), labeling molecules, and overall assay configurations to improve detection sensitivity, specificity, and automated interpretation of results. In most commercial applications, LFIA has been used with enriched food/environmental samples to ensure detection of live cells thus prolonging the assay time to 24-48 h; however, with the recent improvement in LFIA sensitivity, results can be obtained in less than 8 h with shortened and improved enrichment practices. Incorporation of surface-enhanced Raman spectroscopy and/or immunomagnetic separation could significantly improve LFIA sensitivity for near-real-time point-of-care detection of L. monocytogenes for food safety and public health applications.

Automated summary

The use of lateral flow immunoassay (LFIA) and nucleic acid-based lateral flow immunoassay (NALFIA) can improve the sensitivity and specificity of Listeria monocytogenes detection, accelerating the testing process. By improving the ligands and configuration of assay kits, the detection sensitivity and automated interpretation of results in LFIA can be enhanced. Integration of surface-enhanced Raman spectroscopy and immunomagnetic separation technologies can significantly improve the sensitivity of LFIA for rapid food safety monitoring.