A novel copper-based metal-organic framework as a peroxidase-mimicking enzyme and its glucose chemiluminescence sensing application

A novel copper-based metal-organic framework (Cu-MOF) with a large specific surface area and high porosity was synthesized. The Cu-MOF was a good peroxidase-mimicking enzyme and showed a high affinity with hydrogen peroxide in a wide pH range. The catalytic mechanism of Cu-MOF has been studied further based on comparing the characteristic of the Cu-MOF with some isomorphic MOFs. The catalytic activity center of Cu-MOF was determined to be the cupric ion rather than the ligand, which effectively promoted the generation of free radicals and electron transfer in the reaction progress. The high affinity of Cu-MOF to hydrogen peroxide proved it as an ideal catalyst for the chemiluminescence (CL) reaction involving hydrogen peroxide. Therefore, the CL method with high sensitivity could be established for detecting various substrates. A double-enzyme CL glucose biosensing platform was constructed for the determination of serum glucose employing the peroxidase-mimicking properties of Cu-MOF as well as glucose oxidase (GOx).

Automated summary

The newly synthesized Cu-based metal-organic framework (Cu-MOF) exhibited high catalytic activity as a peroxidase-mimicking enzyme and demonstrated strong affinity towards hydrogen peroxide. The catalytic mechanism of Cu-MOF, primarily driven by cupric ions, was found to efficiently generate free radicals and facilitate electron transfer during reactions. Its high affinity for hydrogen peroxide made Cu-MOF an ideal catalyst for chemiluminescence reactions, thus enabling the development of a sensitive detection method for various substrates.