4.3 Article

Vascular effects of disrupting endothelial mTORC1 signaling in obesity

Publisher

AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpregu.00113.2021

Keywords

endothelial dysfunction; high-fat diet; mTORC1; obesity

Categories

Funding

  1. National Institutes of Health [HL084207, UL1TR002537, P30CA086862]
  2. Department of Veterans Affairs [BX004249]
  3. American Heart Association [16POST30830004, 13SDG143400012]
  4. University of Iowa Fraternal Order of Eagles Diabetes Research Center
  5. Carver College of Medicine
  6. Holden Comprehensive Cancer Center
  7. Iowa City Veteran's Administration Medical Center
  8. NIH/NCI [P30CA086862]
  9. University of Iowa Carver College of Medicine

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The study suggests that obesity leads to increased vascular mTORC1 signaling, but this activation does not seem to be necessary for the development of endothelial dysfunction in obesity.
The mechanistic target of rapamycin complex 1 (mTORC1) signaling complex is emerging as a critical regulator of cardiovascular function with alterations in this pathway implicated in cardiovascular diseases. In this study, we used animal models and human tissues to examine the role of vascular mTORC1 signaling in the endothelial dysfunction associated with obesity. In mice, obesity induced by high-fat/high-sucrose diet feeding for similar to 2 mo resulted in aortic endothelial dysfunction without appreciable changes in vascular mTORC1 signaling. On the other hand, chronic high-fat diet feeding (45% or 60% kcal: similar to 9 mo) in mice resulted in en-dothelial dysfunction associated with elevated vascular mTORC1 signaling. Endothelial cells and visceral adipose vessels isolated from obese humans display a trend toward elevated mTORC1 signaling. Surprisingly, genetic disruption of endothelial mTORC1 signaling through constitutive or tamoxifen inducible deletion of endothelial Raptor (critical subunit of mTORC1) did not prevent or rescue the endothelial dysfunction associated with high-fat diet feeding in mice. Endothelial mTORC1 deficiency also failed to reverse the endothelial dysfunction evoked by a high-fat/high-sucrose diet in mice. Taken together, these data show increased vascular mTORC1 signaling in obesity, but this vascular mTORC1 activation appears not to be required for the development of endothelial impairment in obesity.

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