Journal
ADVANCED MATERIALS
Volume 33, Issue 37, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adma.202103221
Keywords
adeno-associated virus; COVID-19; direct electron detector; liquid-phase electron microscopy; SARS-CoV-2; silicon nitride
Categories
Funding
- National Cancer Institute [R01CA193578, R01CA227261, R01CA219700]
- National Institutes of Health
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Liquid-phase electron microscopy (LP-EM) is a new exciting field in materials imaging, offering unprecedented views of molecular processes. Insights from LP-EM studies complement other high-resolution techniques, and expanding this technology into 3D realm presents future opportunities.
Liquid-phase electron microscopy (LP-EM) is an exciting new area in the materials imaging field, providing unprecedented views of molecular processes. Time-resolved insights from LP-EM studies are a strong complement to the remarkable results achievable with other high-resolution techniques. Here, the opportunities to expand LP-EM technology beyond 2D temporal assessments and into the 3D regime are described. The results show new structures and dynamic insights of human viruses contained in minute volumes of liquid while acquired in a rapid timeframe. To develop this strategy, adeno-associated virus (AAV) is used as a model system. AAV is a well-known gene therapy vehicle with current applications involving drug delivery and vaccine development for COVID-19. Improving the understanding of the physical properties of biological entities in a liquid state, as maintained in the human body, has broad societal implications for human health and disease.
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