Journal
ADVANCED FUNCTIONAL MATERIALS
Volume 31, Issue 45, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.202105452
Keywords
aggregation-induced emission; deep-red cell imaging; hypochlorite detection; ratiometric fluorescent probes; squaraine dyes
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Funding
- National Natural Science Foundation of China [21975085, 21704030]
- Innovation and Talent Recruitment Base of New Energy Chemistry and Device [B21003]
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Developing ratiometric fluorescent hypochlorite probes with strong fluorescence, high sensitivity, and low toxicity remains a challenge. A novel molecular design strategy transforms traditional dyes into highly sensitive probes, enabling successful imaging in living cells.
Development of ratiometric fluorescent hypochlorite probes with strong long wavelength fluorescence in aqueous medium, high resistance to photobleaching, high sensitivity and selectivity, and low biological toxicity remains a challenge. In this work, a molecular design strategy is proposed that can transform the traditional squaraine dyes (SQs) with aggregation-caused quenching character into aggregation-induced emission (AIE)-active luminogens by functionalizing the end-groups with tetraphenylethylene units and further introducing hydrophilic sulfonate group as the side chains. The resulting TPE-SQ5 not only emits strong deep-red fluorescence with a high quantum yield of 11.0% and high photostability, but more encouragingly can serve as a ratiometric fluorescent hypochlorite probe with high selectivity and sensitivity (detection limit: 5.6 nm), which indeed is the first report for SQs. The detailed sensing mechanism study demonstrates that the oxindole product with sulfonate substitution is responsible for the ratiometric fluorescent response. Furthermore, TPE-SQ5 nanoparticles with high biocompatibility and low cytotoxicity are successfully used for ratiometrically imaging exogenous and endogenous hypochlorite in living cells.
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