4.5 Article

The amoebicidal activity of three substances derived from benzothiazole on Acanthamoeba castellanii cysts and trophozoites and its cytotoxic potentials

Journal

ACTA TROPICA
Volume 220, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.actatropica.2021.105981

Keywords

Acanthamoeba castellanii cytotoxic effect; Ameobicidial effect; 2-Amino-6(trifluoromethoxy)-benzothiazole; 13-Benzothiazole-6-sulfonyl chloride; Riluzole (2-Amino-6-(trifluoromethoxy); benzothiazole)

Funding

  1. Hatay Mustafa Kemal University Scientific Research Projects Coordination Unit [19.M.048]

Ask authors/readers for more resources

The study showed that benzothiazole derivatives have suppressive effects on the reproductive potential of Acanthamoeba parasites, with 2-Amino-6(trifluoromethoxy)-benzothiazole being the most effective. This substance may be a promising new therapeutic agent for diseases caused by Acanthamoeba parasites, as supported by potential animal experiments.
Acanthamoeba species are free-living amoebae isolated from many ecological areas such as swimming pools, dams, lakes, soil, and air filters. These amoebae are usually causing granulomatous amebic encephalitis and amebic keratitis in immunosuppressive individuals. In this study, the reproductive potential and morphological changes determined of Acanthamoeba castellanii trophozoite and cyst forms exposed to three different active substances derived from benzothiazole. Furthermore, the cytotoxic potential of these active substances determined by XTT analysis. In the study, axenic cultures prepared for Acanthamoeba castellanii cyst and trophozoite forms and parasite exposed to different concentrations of active substances. Cell counts of parasite cultures were performed at the 30 minutes, 1st, 6th, 12th, 24th, and 48th hour periods. As a result of the study, the reproductive potential suppressive effects of all three substances on Acanthamoeba castellanii trophozoites and cysts were determined. The most effective of these substances was 2-Amino-6(trifluoromethoxy)-benzothiazole. In the first three concentrations of this substance (0.1%, 0.05%, 0.025%), no determined trophozoite and cysts at the end of twenty four. Due to its strong ameobicidal effect, it is thought that 2-Amino-6(trifluoromethoxy)-benzothiazole may be a new therapeutic agent in diseases caused by acanthamoeba parasites by supporting this study with animal experiments.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available