Journal
ACS NANO
Volume 15, Issue 7, Pages 11753-11761Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.1c02556
Keywords
nanoparticle; extracellular vesicle; nanoparticle tracking analysis; time-sequential illumination; multifluorescence
Categories
Funding
- National Research Foundation of Korea (NRF) - Korean government (MSIP) [2018R1A2B3006280]
- Korea Medical Device Development Fund - Korean government (Ministry of Science and ICT) [202011B12]
- Korea Medical Device Development Fund - Korean government (Ministry of Health Welfare) [202011B12]
- Korea Medical Device Development Fund - Korean government (Ministry of Food and Drug Safety) [202011B12]
- ExosomePlus, Inc., Republic of Korea
- Korea Medical Device Development Fund - Korean government (Ministry of Trade, Industry and Energy) [202011B12]
- National Research Foundation of Korea [2018R1A2B3006280] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
The study demonstrates a fluorescence-based nanoparticle tracking analysis (NTA) system for characterizing the size and membrane protein expression of individual extracellular vesicles (EVs). By comparing scattering and fluorescent images, membrane proteins labeled on EVs can be detected.
We demonstrate a fluorescence-based nanoparticle tracking analysis (NTA) system for the characterization of both the size and membrane protein expression of individual extracellular vesicles (EVs). A sheet of lasers with four different wavelengths was sequentially shone onto extracellular vesicles according to a preprogrammed schedule, providing scattering images intercalated by three fluorescent images. The presence of extracellular vesicles was tracked frame by frame from scattering images. Fluorescence-labeled membrane proteins on EVs were detected by comparing scattering and fluorescent images. The tetraspanins (CD9, CD63, and CD81) of individual HEK293 EVs analyzed by both NTA and total internal reflection fluorescence microscopy showed that the proposed NTA system can contribute to the understanding of individual extracellular vesicles.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available