Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 13, Issue 33, Pages 38931-38946Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.1c09000
Keywords
hybridization chain reaction; nonlinear assembly; electrochemistry; fluorescence; biosensing; nucleic acids
Funding
- Science and Technology Cooperation Project between the Chinese and Australian Governments [2017YFE0132300, ACSRF65827]
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This article summarizes the assembly principles and applications of hybridization chain reaction (HCR) in DNA nanotechnology. HCR has excellent assembly kinetics and ease of operation, making it suitable for ultra-sensitive detection of various targets. Future advancements in overcoming challenges are needed for further development in this field.
With the continuous development of DNA nanotechnology, various spatial DNA structures and assembly techniques emerge. Hybridization chain reaction (HCR) is a typical example with exciting features and bright prospects in biosensing, which has been intensively investigated in the past decade. In this Spotlight on Applications, we summarize the assembly principles of conventional HCR and some novel forms of linear/nonlinear HCR. With advantages like great assembly kinetics, facile operation, and an enzyme-free and isothermal reaction, these strategies can be integrated with most mainstream reporters (e.g., fluorescence, electrochemistry, and colorimetry) for the ultrasensitive detection of abundant targets. Particularly, we select several representative studies to better illustrate the novel ideas and performances of HCR strategies. Theoretical and practical utilities are confirmed for a range of biosensing applications. In the end, a deep discussion is provided about the challenges and future tasks of this field.
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