Circulating miR-155 and JAK2/STAT3 Axis in Acute Ischemic Stroke Patients and Its Relation to Post-Ischemic Inflammation and Associated Ischemic Stroke Risk Factors

Background: Micro RNAs and their target genes recently have been identified to play a crucial role in the molecular pathogenesis of post-stroke ischemic cellular injury, which elucidates their new role in ischemic stroke diagnosis and therapy. Thus, we evaluated the relative serum expression of miR-155, an inflammatory micro RNA, and the mRNAs (JAK2/STAT3) in acute ischemic stroke patients and its associations with the inflammatory cytokine TNF-alpha and different stroke risk factors. Subjects and Methods: The relative expression of serum miR-155 and mRNAs (JAK2/STAT3) was assessed using RT-PCR, serum TNF-alpha was measured using ELIZA in 46 acute ischemic stroke patients and 50 control subjects. Receiver operating characteristic (ROC) curve was constructed to assess the specificity and sensitivity of circulating miR-155, JAK2/STAT3 as biomarkers for acute ischemic stroke. Results: Circulating miR-155, JAK2/STAT3 were significantly up-regulated among stroke patients (8.5, 2.9, 4.2 fold respectively, P<0.001) with significant increase in TNF-alpha (263.8 +/- 10.7 pg/mL, P <0.001). MiR-155, JAK2/STAT3 were positively correlated with TNF-alpha. MiR-155, JAK2/STAT3 were significantly increased in stroke patients and associated with risk factors such as hypertension, carotid atherosclerosis, and atrial fibrillation. Our study revealed that miR-155 has diagnostic accuracy for acute ischemic stroke where AUC=0.9, (P<0 .001 ). Conclusion: The elevated expressions of circulating miR-155, JAK2/STAT3, and TNF-alpha in acute ischemic stroke patients could trigger post-stroke cellular inflammation. MiR-155 could be used as potential inflammatory biomarker for acute ischemic stroke. However, further clinical studies are still needed to determine the exact role of miRNAs and different signal transduction expressions in the stage of acute ischemic stroke.

Automated summary

The study found that in patients with acute ischemic stroke, circulating expressions of miR-155, JAK2/STAT3, and TNF-alpha are elevated, potentially triggering post-stroke cellular inflammation and associated with certain risk factors. MiR-155 could serve as a potential inflammatory biomarker for acute ischemic stroke. Further clinical studies are needed to determine the exact role of miRNAs and different signal transduction expressions in the acute ischemic stroke stage.