Journal
BIOMEDICINES
Volume 9, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/biomedicines9040400
Keywords
skin derived precursors; hair follicle neogenesis; engineered skin substitute
Categories
Funding
- Natural Science Foundation of China [31961160702, 81903239]
- National Key R&D Program of China [2017YFC1103304]
- Shenzhen Science and Technology Innovation Committee [JCYJ20180307123901314, JCYJ20190809180217220, JCYJ20180307123807944]
- State Key Laboratory of Chemical Oncogenomics fund, China Postdoctoral Science Foundation [2019M663673]
- Tsinghua Shenzhen International Graduate School Overseas Cooperation Fund [HW2018006]
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SKPs can serve as the hair-inductive cells in engineered skin substitutes, providing them with the potential for hair follicle regeneration.
Currently, engineered skin substitutes (ESS) are unable to regenerate cutaneous appendages. Recent studies have shown that skin-derived precursors (SKPs), which are extensively available, have the potential to induce hair follicle neogenesis. Here, we demonstrate that ESS consisting of culture-expanded SKPs and epidermal stem cells (Epi-SCs) reconstitute the skin with hair follicle regeneration after grafting into nude mice. SKPs seeded in a C-GAG matrix proliferated and expressed higher levels of hair induction signature genes-such as Akp2, Sox2, CD133 and Bmp6-compared to dermal fibroblasts. Moreover, when ESS prepared by seeding a mixture of culture-expanded murine SKPs and human adult Epi-SCs into a C-GAG matrix was grafted into full-thickness skin wounds in nude mice, black hairs were generated within 3 weeks. Immunofluorescence analysis showed that the SKPs were localized to the dermal papillae of the newly-formed hair follicle. Our results indicate that SKPs can serve as the hair-inductive cells in ESS to furnish it with hair genesis potential
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