Journal
PLANTS-BASEL
Volume 10, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/plants10040705
Keywords
quantitative trait locus (QTL); sequencing based genotyping; RAD-seq; anaerobic germination (AG); rice (Oryza sativa)
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Funding
- German Federal Ministry for Economic Cooperation and Development (BMZ) [81157485]
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The study focused on genetic basis of tolerance to anaerobic germination (AG) in the landrace ASD1 from India, identifying two significant quantitative trait loci (QTLs) on chromosomes 7 and 9 (qAG7 and qAG9) using RAD-seq technology and high-density SNP markers. This high-resolution genetic linkage map may aid in further dissecting the genetic factors of these QTLs.
Direct seeded rice (DSR) is a mainstay for planting rice in the Americas, and it is rapidly becoming more popular in Asia. It is essential to develop rice varieties that are suitable for this type of production system. ASD1, a landrace from India, possesses several traits desirable for direct-seeded fields, including tolerance to anaerobic germination (AG). To map the genetic basis of its tolerance, we examined a population of 200 F-2:3 families derived from a cross between IR64 and ASD1 using the restriction site-associated DNA sequencing (RAD-seq) technology. This genotyping platform enabled the identification of 1921 single nucleotide polymorphism (SNP) markers to construct a high-resolution genetic linkage map with an average interval of 0.9 cM. Two significant quantitative trait loci (QTLs) were detected on chromosomes 7 and 9, qAG7 and qAG9, with LOD scores of 7.1 and 15.0 and R-2 values of 15.1 and 29.4, respectively. Here, we obtained more precise locations of the QTLs than traditional simple sequence repeat and low-density SNP genotyping methods and may help further dissect the genetic factors of these QTLs.
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