Journal
PLANTS-BASEL
Volume 10, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/plants10040758
Keywords
AGL15; MADS-box gene; Arabidopsis thaliana; EAR motif; repression; somatic embryogenesis; seed
Categories
Funding
- National Science Foundation [IOS-1656380]
- National Institute of Food and Agriculture, U.S. Department of Agriculture, Hatch project [1013409]
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AGL15, a member of the MADS domain family of transcription factors, can promote somatic embryogenesis through its ability to repress gene expression, although alterations in its structure may affect its function.
AGAMOUS-like 15 (AGL15) is a member of the MADS domain family of transcription factors (TFs) that can directly induce and repress target gene expression, and for which promotion of somatic embryogenesis (SE) is positively correlated with accumulation. An ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motif of form LxLxL within the carboxyl-terminal domain of AGL15 was shown to be involved in repression of gene expression. Here, we examine whether AGL15 ' s ability to repress gene expression is needed to promote SE. While a form of AGL15 where the LxLxL is changed to AxAxA can still promote SE, another form with a strong transcriptional activator at the carboxy-terminal end, does not promote SE and, in fact, is detrimental to SE development. Select target genes were examined for response to the different forms of AGL15.
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