Antagonistic activity of Trichoderma harzianum and Trichoderma viride strains against some fusarial pathogens causing stalk rot disease of maize, in vitro

Objectives: A high incidence of stalk rot disease of maize causes huge economic losses as well as deleterious effects on the environment and human health resulting from fungicide use. Against this background, the current study was established to evaluate the antagonistic efficacy of Trichoderma viride and Trichoderma harzianum strains against Fusarium proliferatum and Fusarium verticillioides strains, the most common causative agents of stalk rot disease of maize. Methods: Dual culture assay was performed to determine the antagonistic efficacy of Trichoderma strains against some fusarial pathogens of maize. Mycoparasitic relationships of the antagonistic fungal strains against fungal pathogens were investigated using a slide culture technique. Furthermore, a food poisoning technique was performed to detect the antimycotic efficacy of Trichoderma culture filtrates against fusarial pathogens of maize. Antifungal activity of organic solvent extracts of T. harzianum and T. viride was evaluated using the disc diffusion method. GC-MS analysis was used to detect the active components of these extracts. Results and conclusion: Trichoderma viride showed antagonistic activity against F. proliferatum and F. verticillioides with mycelial inhibition rates of 80.17% and 70.46%, while T. harzianum exhibited rates of 68.38% and 60.64%, respectively. The culture filtrates of T. viride and T. harzianum strains exhibited anti fungal activity against F. verticillioides strain with suppressive rates of 56.7% and 32.2%, while the mycelial inhibition rates against F. proliferatum strain were 44.09% and 23.50%, respectively. Mycoparasitic action of T. harzianum strain against fusarial strains was detected, while no mycoparasitism was observed between T. viride and fungal pathogens. The fungicidal concentration of carbendazim fungicide against F. proliferatum was 2.00 ppm, while F. verticillioides strain exhibited resistance to carbendazim fungicide. Moreover, the acetonic extracts of T. viride and T. harzianum strains exerted the highest antifungal potency against F. proliferatum strain, recording minimum inhibitory concentrations of 0.25 and 0.50 mg/ml, respectively. The main bioactive constituents of the acetonic extracts of T. viride and T. harzianum strains were palmitic acid (22.87%) and acetic acid (21.36%), respectively. In conclusion, the antagonistic strains could be a potential source of novel biological fungicides, especially against carbendazim-resistant F. verticillioides strain, avoiding side effects of chemical fungicides. (c) 2021 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Automated summary

The study evaluated the antagonistic efficacy of Trichoderma viride and Trichoderma harzianum strains against the major causative agents of stalk rot disease of maize, with Trichoderma viride showing higher antifungal activity. Mycoparasitic action of T. harzianum against fungal pathogens was detected, while no mycoparasitism was observed for T. viride against them.