Journal
ANTIBIOTICS-BASEL
Volume 10, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/antibiotics10040395
Keywords
Staphylococcus aureus; MRSA; spa typing; MLST; SCCmec typing; clonal analysis; epidemiology
Categories
Funding
- Ministry of Health, Czech Republic-conceptual development of research organization the National Institute of Public Health-NIPH [75010330]
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This study aimed to characterize methicillin-resistant Staphylococcus aureus (MRSA) isolates from the blood of hospitalized patients in the Czech Republic between 2016 and 2018. The majority of MRSA strains were found to be resistant to several antibiotics, with 52 different spa types identified in the analyzed strains. The CC5 clonal complex was predominant in this study, with other CCs such as CC398, CC22, CC8, CC45, and CC97 also present.
Staphylococcus aureus is one of the major causes of bloodstream infections. The aim of our study was to characterize methicillin-resistant Staphylococcus aureus (MRSA) isolates from blood of patients hospitalized in the Czech Republic between 2016 and 2018. All MRSA strains were tested for antibiotic susceptibility, analyzed by spa typing and clustered using a Based Upon Repeat Pattern (BURP) algorithm. The representative isolates of the four most common spa types and representative isolates of all spa clonal complexes were further typed by multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing. The majority of MRSA strains were resistant to ciprofloxacin (94%), erythromycin (95.5%) and clindamycin (95.6%). Among the 618 strains analyzed, 52 different spa types were detected. BURP analysis divided them into six different clusters. The most common spa types were t003, t586, t014 and t002, all belonging to the CC5 (clonal complex). CC5 was the most abundant MLST CC of our study, comprising of 91.7% (n = 565) of spa-typeable isolates. Other CCs present in our study were CC398, CC22, CC8, CC45 and CC97. To our knowledge, this is the biggest nationwide study aimed at typing MRSA blood isolates from the Czech Republic.
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