Journal
FOODS
Volume 10, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/foods10040883
Keywords
Hemerocallis citrina Baroni; liquid chromatography coupled with mass spectrometry sleep; Drosophila melanogaster; network pharmacology; mRNA expression
Categories
Funding
- Key Realm R&D Program of Guangdong Province [2019B020211002]
- National Natural Science Foundation of China [31972157]
- Science and Technology Planning Project of Guangzhou City [201804020077]
- International Cooperation Program of SCAU [2019SCAUGH03]
- Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme
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Research on the sleep-improvement effects of Hemerocallis citrina Baroni (HC) in Drosophila melanogaster revealed that HC hydroalcoholic extract could regulate sleep and the main bioactive components were identified as quercetin, luteolin, kaempferol, caffeic acid, and nicotinic acid. The core targets affected by HC included Akt1, Cat, Ple, and Sod, highlighting the multi-component and multi-target features of HC in sleep-improvement.
Hemerocallis citrina Baroni (HC) is an edible plant in Asia, and it has been traditionally used for sleep-improvement. However, the bioactive components and mechanism of HC in sleep-improvement are still unclear. In this study, the sleep-improvement effect of HC hydroalcoholic extract was investigated based on a caffeine-induced insomnia model in Drosophila melanogaster (D. melanogaster), and the ultrahigh-performance liquid chromatography coupled with electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC-ESI-Orbitrap-MS) and network pharmacology strategy were further combined to screen systematically the active constituents and mechanism of HC in sleep-improvement. The results suggested HC effectively regulated the number of nighttime activities and total sleep time of D. melanogaster in a dose-dependent manner and positively regulated the sleep bouts and sleep duration of D. melanogaster. The target screening suggested that quercetin, luteolin, kaempferol, caffeic acid, and nicotinic acid were the main bioactive components of HC in sleep-improvements. Moreover, the core targets (Akt1, Cat, Ple, and Sod) affected by HC were verified by the expression of the mRNA of D. melanogaster. In summary, this study showed that HC could effectively regulate the sleep of D. melanogaster and further clarifies the multi-component and multi-target features of HC in sleep-improvement, which provides a new insight for the research and utilization of HC.
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