Journal
METABOLITES
Volume 11, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/metabo11050294
Keywords
lipidomics; lipids; sample preparation; SPE; mass spectrometry; liquid chromatography
Categories
Funding
- Agilent
Ask authors/readers for more resources
This article introduces a novel solid-phase extraction method for extracting lipids from human plasma, which saves time and labor compared to traditional liquid-liquid extraction methods, and provides equivalent or better qualitative and quantitative results in terms of recovery, reproducibility, and lipidome coverage.
Lipidomic approaches are widely used to investigate the relationship between lipids, human health, and disease. Conventional sample preparation techniques for the extraction of lipids from biological matrices like human plasma are based on liquid-liquid extraction (LLE). However, these methods are labor-intensive, time-consuming, and can show poor reproducibility and selectivity on lipid extraction. A novel, solid-phase extraction (SPE) approach was demonstrated to extract lipids from human plasma using a lipid extraction SPE in both cartridge and 96-well-plate formats, followed by analysis using a combination of targeted and untargeted liquid chromatography/mass spectrometry. The Lipid Extraction SPE method was compared to traditional LLE methods for lipid class recovery, lipidome coverage, and reproducibility. The novel SPE method used a simplified protocol with significant time and labor savings and provided equivalent or better qualitative and quantitative results than traditional LLE methods with respect to several critical performance metrics; recovery, reproducibility, and lipidome coverage.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available