Journal
METABOLITES
Volume 11, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/metabo11040202
Keywords
stable isotope tracers; Nuclear Magnetic Resonance; lipid C-13 incorporation; isotopomer distributions
Categories
Funding
- NCI Center for Cancer Research [1R01ES022191-01, P01CA163223-01A1, R21 ES025669-01, P30 CA177558, 1U24DK097215-01A1]
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Lipids are crucial for cellular function as they serve various roles, but their chemical diversity and dynamic range in cells make detailed analysis complex. Stable isotope tracers can help determine lipid synthesis and degradation, but increase the number of distinguishable molecules. NMR analysis can add value in global lipid analysis and isotopomer distributions.
Lipids comprise diverse classes of compounds that are important for the structure and properties of membranes, as high-energy fuel sources and as signaling molecules. Therefore, the turnover rates of these varied classes of lipids are fundamental to cellular function. However, their enormous chemical diversity and dynamic range in cells makes detailed analysis very complex. Furthermore, although stable isotope tracers enable the determination of synthesis and degradation of complex lipids, the numbers of distinguishable molecules increase enormously, which exacerbates the problem. Although LC-MS-MS (Liquid Chromatography-Tandem Mass Spectrometry) is the standard for lipidomics, NMR can add value in global lipid analysis and isotopomer distributions of intact lipids. Here, we describe new developments in NMR analysis for assessing global lipid content and isotopic enrichment of mixtures of complex lipids for two cell lines (PC3 and UMUC3) using both C-13(6) glucose and C-13(5) glutamine tracers.
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