Journal
MICROORGANISMS
Volume 9, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/microorganisms9051015
Keywords
Cryptosporidium parvum; T-cell immunomodulatory protein (TIP) homolog; type I membrane protein; adhesion protein; binding kinetics; apparent dissociation constant
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Funding
- National Key R&D Program of China [2017YFD0500401]
- National Natural Science Foundation of China [31772731]
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Cryptosporidium parvum is a zoonotic parasite that infects intestinal epithelial cells, causing watery diarrhea. The newly identified CpTIPH protein shows high binding affinity to host cells and is involved in parasite invasion. Further investigation into the role of CpTIPH in parasite-host cell interactions is warranted.
Cryptosporidium parvum is a globally recognized zoonotic parasite of medical and veterinary importance. This parasite mainly infects intestinal epithelial cells and causes mild to severe watery diarrhea that could be deadly in patients with weakened or defect immunity. However, its molecular interactions with hosts and pathogenesis, an important part in adaptation of parasitic lifestyle, remain poorly understood. Here we report the identification and characterization of a C. parvum T-cell immunomodulatory protein homolog (CpTIPH). CpTIPH is a 901-aa single-pass type I membrane protein encoded by cgd5_830 gene that also contains a short Vibrio, Colwellia, Bradyrhizobium and Shewanella (VCBS) repeat and relatively long integrin alpha (ITGA) N-terminus domain. Immunofluorescence assay confirmed the location of CpTIPH on the cell surface of C. parvum sporozoites. In congruence with the presence of VCBS repeat and ITGA domain, CpTIPH displayed high, nanomolar binding affinity to host cell surface (i.e., K-d(App) at 16.2 to 44.7 nM on fixed HCT-8 and CHO-K1 cells, respectively). The involvement of CpTIPH in the parasite invasion is partly supported by experiments showing that an anti-CpTIPH antibody could partially block the invasion of C. parvum sporozoites into host cells. These observations provide a strong basis for further investigation of the roles of CpTIPH in parasite-host cell interactions.
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