Journal
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
Volume 9, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fcell.2021.671989
Keywords
retinal vasculature; choroidal vasculature; endothelial cells; pericytes; astrocytes
Categories
Funding
- Research to Prevent Blindness
- Retina Research Foundation
- RRF/Daniel M. Albert Chair [P30 EY016665, P30 CA014520, R01 EY026078, EY030076]
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TSP1 is a vital regulator of angiogenesis and inflammation in the eye, with its loss contributing to increased retinal vascular density and pathological ocular neovascularization. The study found that while global knockout of TSP1 led to increased retinal vascular density, only lack of TSP1 expression in endothelial cells was sufficient to increase choroidal neovascularization. Individual cell type loss of TSP1 resulted in decreased retinal endothelial cell numbers in a cell type-specific manner, highlighting the unique role TSP1 plays in each cell type.
Tight regulation of positive and negative regulators of angiogenesis is essential, particularly in the eye where their dysregulation can lead to vision loss. Thrombospondin-1 (TSP1) is a matricellular protein that negatively regulates angiogenesis and inflammation in the eye. It aids ocular vascular homeostasis such that its loss contributes to increased retinal vascular density and pathologic ocular neovascularization. Our previous studies demonstrated that mice globally lacking TSP1 expression had increased retinal vascular density, decreased hyperoxia-induced retinal vessel loss, and increased choroidal neovascularization. Here we determined the impact to the ocular vasculature of endothelial cell, pericyte, or astrocyte loss of TSP1 expression. Only lack of TSP1 expression in endothelial cells was sufficient to increase choroidal neovascularization with mice lacking expression in pericytes or astrocytes not demonstrating a significant impact. Although the global TSP1 knockout mice demonstrated increased retinal vascular density, individual cell type loss of TSP1 resulted in decreased retinal endothelial cell numbers before and/or after vascular maturation in a cell type specific fashion. Retinas from mice lacking TSP1 expression in endothelial cells, pericytes or astrocytes were not protected from retinal vessel regression in response to hyperoxia as we previously observed in the global knockout. Thus, modulation of TSP1 expression in individual cell types demonstrates a response that is unique to the role TSP1 plays in that cell type of interest, and their coordinated activity is critical for vision.
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