Journal
AGRONOMY-BASEL
Volume 11, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/agronomy11040789
Keywords
DNA methylation; promoter; dsRNA; siRNAs; RNAi; bisulfite sequencing
Categories
Funding
- European Regional Development Fund (ERDF), through the Operational Program Southern Aegean [NAIG1-0040991]
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Exogenous RNA molecules can trigger RNA interference in plants, and high-pressure spraying of dsRNAs can induce de novo methylation of promoter sequences in plants.
Exogenous application of RNA molecules is a potent method to trigger RNA interference (RNAi) in plants in a transgene-free manner. So far, all exogenous RNAi (exo-RNAi) applications have aimed to trigger mRNA degradation of a given target. However, the issue of concomitant epigenetic changes was never addressed. Here, we report for the first time that high-pressure spraying of dsRNAs can trigger de novo methylation of promoter sequences in plants.
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