Canine Placenta Histological Findings and Microvascular Density: The Histological Basis of a Negative Neonatal Outcome?

Simple Summary Accurate examination of the placenta, mainly by gross inspection rather than by histology, is common in clinical practice in some species, for example, in horses, but not carried out routinely in dogs. Placenta alterations in the mare can indicate malfunction, and data exist that intrauterine fetal nutrition influences both perinatal health, but also performances in adult life. Often placenta lesions are not easily appreciable on macroscopical examination, and histology is the best way to assess damage associated with sick newborns. The results of this paper underline the association between placenta injuries and outcome conditions in puppies and confirm, also in dogs, histological placenta investigation as a useful adjunctive tool in the evaluation of the newborn's health and prognosis. Placenta is essential for the development of the fetus, and its impaired function can lead to a negative outcome (i.e., neonatal mortality). In dogs, investigations on placenta histology and neonatal outcome in healthy bitches are lacking, and a contribution is provided in this study to emphasize the use of placenta histology in practice. Fifty-one placentas from 11 litters were collected during cesarean section, classified according to the litter size (large (L) or small (S)) and the outcome, this latter as healthy (Group 1) or dead within 7 days (Group 2). The placenta/puppy weight ratio (PPR) was calculated, and specimens were formalin-fixed and paraffin-wax embedded, and on the resulting histological slides, capillary density (CD) was quantified. Among necrosis, calcification, and intravascular leucocytes, only the presence of multifocal-confluent necrosis (significantly more frequent in Group 2) was associated with a higher risk of death within 7 days (odds ratio = 30.7). Mixed logistic regression ruled out the effect on death both of a bitch and cesarean type (programmed vs. emergency). PPR and CD values were associated with litter size; large litters had lower PPR (p < 0.01) and higher CD (p < 0.05) than small litters. The relationship between PPR and CD was negative and significant (p < 0.01). Necrosis was a frequent finding in canine placentas, but only when multifocal-confluent was it associated with a poor outcome. The litter size influenced PPR (lower in L) and CD (higher in L), and this is likely due to the plasticity of placenta adaptation.

Automated summary

This study highlights the association between placenta injuries and outcome conditions in newborn puppies, confirming the usefulness of histological placenta investigation in evaluating newborn health and prognosis. The function of the placenta is crucial for fetal development, and its impairment can lead to negative outcomes.