4.1 Article

Vitis vinifera VvPUB17 functions as a E3 ubiquitin ligase and enhances powdery mildew resistance via the salicylic acid signaling pathway

Journal

JOURNAL OF BERRY RESEARCH
Volume 11, Issue 3, Pages 419-430

Publisher

IOS PRESS
DOI: 10.3233/JBR-210709

Keywords

Disease resistance; E3 ubiquitin ligase; grapevine (Vitis vinifera); plant U-box (PUB); powdery mildew; salicylic acid

Funding

  1. National Natural Science Foundation of China [31701893]
  2. Key R&D and Promotion Projects in Henan Province [202102110043]
  3. Training Project for Young Backbone Teachers of Colleges and Universities of Henan Province [81]
  4. Program for Science & Technology Innovation Talents in Universities of Henan Province [21HASTIT035]
  5. Program for Innovative Research Team (in Science and Technology) in University of Henan Province [21IRTSTHN021]
  6. Leading Talents Found in Science and Technology Innovation in Henan Province [194200510007]

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The study revealed that the VvPUB17 gene enhances grapevine resistance to powdery mildew through the SA signal pathway. Transgenic plants with elevated levels of key genes related to the SA defense pathway and high concentrations of salicylic acid exhibited increased resistance to powdery mildew.
BACKGROUND: Powdery mildew affects grapevine growth and development and reduces grapevine fruit yield and quality. Plant U-box (PUB) E3 ubiquitin ligases play important roles in ubiquitin/proteasome-mediated protein degradation during plant development and in the plant defense response. OBJECTIVE: We cloned the VvPUB17 gene from Vitis vinifera and analyzed that VvPUB17 enhanced the resistance of grapevine to powdery mildew through the SA signal pathway. METHODS: Pathogen inoculation of Arabidopsis thaliana and grapevine plants was carried out by the tableting method. Gene expression was analyzed by quantitative real-time PCR. Sequence analysis and in vitro ubiquitination experiments show the structure and characteristics of VvPUB17. Exogenous salicylic acid, methyl jasmonate, ethylene and powdery mildew induced the expression of VvPUB17 in Arabidopsis leaves to verify the resistance of VvPUB17 to powdery mildew. RESULTS: Sequence analysis and in vitro ubiquitination experiments show that VvPUB17 contains U-box and Armadillo repeats (ARM repeat) and has E3 ubiquitin ligase activity dependent on the conserved U-box motif. Transgenic plants showed elevated levels of key genes related to the SA defense response pathway and high concentrations of salicylic acid. CONCLUSIONS: VvPUB17 functions as an E3 ubiquitin ligase that enhances the resistance of grapes to powdery mildew through the SA signal pathway.

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