Journal
INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE
Volume 14, Issue -, Pages 68-74Publisher
ELSEVIER
DOI: 10.1016/j.ijppaw.2020.12.003
Keywords
Trypanosomatid; Isolation; Low-passage; Lotmaria passim; Honeybee; Experimental infection
Categories
Funding
- Spanish Programme for Knowledge Generation and Scientific and Technological Strengthening of the R + D + I System: Generacion del Conocimiento 2018 [PGC2018-098929-A-I00]
- [INIA ERTA2014-00003]
Ask authors/readers for more resources
The study revealed the impact of cell culture passages on the pathogenicity of L. passim in honeybees, with the newly obtained C1 strain showing higher virulence, providing further evidence of its negative effects on honeybee health.
The impact of trypanosomatid parasites on honeybee health may represent a major threat to bee colonies worldwide. However, few axenic isolates have been generated to date and with no details on cell culture passages, a parameter that could influence parasite virulence. To address this question, a trypanosomatid isolation protocol was developed and a new strain was obtained, named L. passim C1. Using experimental infection of worker honeybees, we compared the virulence and mortality rates of the ATCC PRA-403 reference strain and C1 strain, the latter showing higher virulence from 10 days post-infection onward. This study highlights the impact of cell culture passages on the pathogenicity of L. passim in honeybees, providing new evidence of its negative effects on honeybee health.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available