Journal
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
Volume 19, Issue -, Pages 3042-3050Publisher
ELSEVIER
DOI: 10.1016/j.csbj.2021.05.026
Keywords
Haemophilus influenzae; Airway infection; Genome; Transcriptome; Methylome; Proteome; Metabolome; Tn-seq
Funding
- Regional Navarra Govern, Spain [0011-1408-2017-000000]
- Agencia Espanola de Investigacion (AEI), Spain [PRE2019-088382]
- MINECO [RTI2018-096369-B-I00]
- SEPAR [875/2019]
- Gobierno de Navarra [PC150-151-152]
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Haemophilus influenzae has played a significant role in key bacterial genome sequencing milestones, contributing to our understanding of the human-adapted pathogen responsible for respiratory infections. The evolving technologies have led to insights into essential genes, virulence factors, and gene regulatory networks, posing challenges in understanding genomic plasticity and identifying clinical targets. Multi-omics approaches have greatly enhanced our knowledge of the interactions between H. influenzae and the human airways, offering strategies for biomedical research and clinical applications.
Haemophilus influenzae has contributed to key bacterial genome sequencing hallmarks, as being not only the first bacterium to be genome-sequenced, but also starring the first genome-wide analysis of chromosomes directly transformed with DNA from a divergent genotype, and pioneering Tn-seq methodologies. Over the years, the phenomenal and constantly evolving development of -omic technologies applied to a whole range of biological questions of clinical relevance in the H. influenzae-host interplay, has greatly moved forward our understanding of this human-adapted pathogen, responsible for multiple acute and chronic infections of the respiratory tract. In this way, essential genes, virulence factors, pathoadaptive traits, and multi-layer gene expression regulatory networks with both genomic and epigenomic complexity levels are being elucidated. Likewise, the unstoppable increasing whole genome sequencing information underpinning H. influenzae great genomic plasticity, mainly when referring to noncapsulated strains, poses major challenges to understand the genomic basis of clinically relevant phenotypes and even more, to clearly highlight potential targets of clinical interest for diagnostic, therapeutic or vaccine development. We review here how genomic, transcriptomic, proteomic and metabolomicbased approaches are great contributors to our current understanding of the interactions between H. influenzae and the human airways, and point possible strategies to maximize their usefulness in the context of biomedical research and clinical needs on this human-adapted bacterial pathogen. (C) 2021 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology.
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