Journal
JOURNAL OF CLINICAL MEDICINE
Volume 10, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/jcm10081570
Keywords
Candida; MALDI– TOF MS; HIV; oral cavity fungal flora
Categories
Funding
- Wroclaw Medical University [STM.A130.18.008]
- Students Scientific Society
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This study compared classical microbiological methods with MALDI-TOF MS in fungi identification, showing that MALDI-TOF MS is an effective alternative, especially for HIV-positive patients, due to the different morphology of fungal colonies.
Background: A growing incidence of invasive fungal infections, especially among immunocompromised patients, has given increased significance to microbiological diagnostics of yeast-like fungi. More accurate and faster fungi identification methods that can compete with classical methods are being searched for. In this paper, classical microbiological methods are compared to MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry). Methods: The diagnostic material was collected from buccal mucosa from 98 adults, including 69 with HIV. Only positive cultures were included in the study. Results: Matching results were obtained in 45 samples, and there were nonmatching results in 35 samples, with the majority of these in the study group, constituting 50% of identifications within this group. A particularly common mistake resulting from the use of classical methods is the false identification of C. dubliniensis as C. albicans. Additionally, C. tropicalis proves to be difficult to identify. Conclusions: Our results and literature data suggest that MALDI-TOF MS should be considered an effective alternative to classical methods in terms of fungi identification, especially among HIV-positive patients, due to the different morphology of fungal colonies.
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