Journal
JOURNAL OF CLINICAL MEDICINE
Volume 10, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/jcm10071541
Keywords
tympanic membrane; chronic perforation; acellular dermal matrix; epidermal growth factor; basic fibroblast growth factor; regeneration
Categories
Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology [NRF-2018-RID-1A1B07048074]
- National Research Foundation of Korea (NRF) - Ministry of Science and ICT for Bioinspired Innovation Technology Development Project [NRF-2018M3C1B7021997]
- Korea Medical Device Development Fund grant - Korea government (Ministry of Science and ICT) [202013C05]
- Korea Medical Device Development Fund grant - Korea government (Ministry of Trade, Industry and Energy) [202013C05]
- Korea Medical Device Development Fund grant - Korea government (Ministry of Health & Welfare, Republic of Korea) [202013C05]
- Korea Medical Device Development Fund grant - Korea government (Ministry of Food and Drug Safety) [202013C05]
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This study investigated the use of ADM/MSCs combined with growth factors in chronic tympanic membrane transplantation and found that the success rate was significantly higher in the ADM/MSC/EGF group, suggesting that this combination could potentially be used as an outpatient treatment for eardrum regeneration in the future, without the need for surgery.
The success rate of grafting using acellular dermal matrix (ADM) for chronic tympanic membrane was reported in previous studies to be lower than fascia or perichondrium. Combining mesenchymal stem cells (MSCs) and growth factor-loaded ADM for the regeneration of chronic TMP has not been reported so far. In this study, we hypothesized that combining growth factor-loaded ADM/MSCs could promote the recruitment of MSCs and assist in TMP regeneration. We evaluated the regeneration and compared the performance of four scaffolds in both in vitro and in vivo studies. MTT, qPCR, and immunoblotting were performed with MSCs. In vivo study was conducted in 4 groups (control; ADM only, ADM/MSC, ADM/MSC/bFGF, ADM/MSC/EGF) of rats and inferences were made by otoendoscopy and histological changes. Attachment of MSCs on ADM was observed by confocal microscopy. Proliferation rate increased with time in all treated cells. Regeneration-related gene expression in the treated groups was higher. Also, graft success rate was significantly higher in ADM/MSC/EGF group than other groups. Significant relationships were disclosed in neodrum thickness between each group. The results suggest, in future, combining EGF with ADM/MSCs could possibly be used as an outpatient treatment, without the need for surgery for eardrum regeneration.
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