Journal
EXPOSURE AND HEALTH
Volume 13, Issue 3, Pages 465-475Publisher
SPRINGER
DOI: 10.1007/s12403-021-00396-7
Keywords
Polychlorinated biphenyls; Organochlorinated pesticides; Brominated flame retardants; Perfluorinated alkylating agents; Cytotoxicity; High content analysis
Categories
Funding
- Norwegian Research Council (NFR) [213076/H10, 204361/H10]
Ask authors/readers for more resources
Exposure to a total mixture of 29 persistent organic pollutants altered mitochondrial metabolism and induced cell death via an alternative mechanism to valinomycin. Specific combinations of individual chemical classes, but not the total mixture, affected cell number.
A total mixture of 29 persistent organic pollutants (POPs) modelled from Scandinavian blood concentrations was used to expose human A-498 kidney cells for 24 h over a concentration range spanning below to above blood level (1/10x, 1x, 50x, 100x, 500x). Its constituent submixtures (PFAA, Br, Cl) and co-mixtures (PFAA + Br, PFAA + Cl, Br + Cl) were also tested. Valinomycin (12 mu M) was used as a cytotoxic comparative compound. Cell number (CN), nuclear area (NA), nuclear intensity (NI), mitochondrial membrane potential (MMP), and mitochondrial mass (MM) were assessed using high content analysis (HCA). Only the co-mixtures (PFAA + Cl, PFAA + Br) at 50x and 50x, 500x decreased CN, respectively. NI was increased by the total mixture at 500x and Cl mixture at all concentrations tested. MMP was increased by the total mixture at 100x and 500x, PFAA at 1x, Br + Cl and PFAA + Cl at 100x and 500x, respectively. MM was decreased by the total mixture at 500x. In contrast, valinomycin decreased CN and surviving cells showed a decrease in MMP and an increase in MM. In conclusion, POP exposure altered mitochondrial metabolism and induced cell death via an alternative mechanism to valinomycin. Only specific combinations of individual chemical classes, but not the total mixture, affected cell number.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available