Journal
FRONTIERS IN CHEMISTRY
Volume 9, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fchem.2021.666867
Keywords
sorting methods; enzyme directed evolution; microfluidics; droplet; high-throughput
Categories
Funding
- National Natural Science Foundation of China [82073911]
- Shandong Provincial Natural Science Foundation [ZR2020MH389]
- Medical and Health Science and Technology Project of Shandong Province [2017WS075, 202011000657]
- Innovation Project of Shandong Academy of Medical Sciences
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Droplet-based microfluidics has been widely used in enzyme directed evolution due to its low cost and high throughput. Various sorting methods, including labeled detections like FADS and label-free detections like ECDS, have been developed to improve the efficiency of the system. Recent advancements in microfluidic DE have led to the generation of novel enzymes and highly efficient variants. Challenges and advantages of different sorting methods have been discussed to provide insights for future applications in enzyme DE.
Droplet-based microfluidics has been widely applied in enzyme directed evolution (DE), in either cell or cell-free system, due to its low cost and high throughput. As the isolation principles are based on the labeled or label-free characteristics in the droplets, sorting method contributes mostly to the efficiency of the whole system. Fluorescence-activated droplet sorting (FADS) is the mostly applied labeled method but faces challenges of target enzyme scope. Label-free sorting methods show potential to greatly broaden the microfluidic application range. Here, we review the developments of droplet sorting methods through a comprehensive literature survey, including labeled detections [FADS and absorbance-activated droplet sorting (AADS)] and label-free detections [electrochemical-based droplet sorting (ECDS), mass-activated droplet sorting (MADS), Raman-activated droplet sorting (RADS), and nuclear magnetic resonance-based droplet sorting (NMR-DS)]. We highlight recent cases in the last 5 years in which novel enzymes or highly efficient variants are generated by microfluidic DE. In addition, the advantages and challenges of different sorting methods are briefly discussed to provide an outlook for future applications in enzyme DE.
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