Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase

Deamination of cytosine and dUMP misincorporation have been found to be capable of producing uracil in the genome. This study presents the Al-seq (artificial incorporation modified nucleobase for sequencing), a base substitution, which not only is capable of profiling uracil at single-nucleotide resolution and showing its centromeric enrichment but could also reveal that the identified uracil sites are derived from cytosine deamination. All the results indicate the potential biological significance of uracil as the epigenetic modification.

Automated summary

The study introduces Al-seq as a method to profile uracil at single-nucleotide resolution and show its centromeric enrichment, revealing the biological significance of uracil as an epigenetic modification derived from cytosine deamination.