4.6 Article

An RNA Triangle with Six Ribozyme Units Can Promote a Trans-Splicing Reaction through Trimerization of Unit Ribozyme Dimers

Journal

APPLIED SCIENCES-BASEL
Volume 11, Issue 6, Pages -

Publisher

MDPI
DOI: 10.3390/app11062583

Keywords

catalytic RNA; group I ribozyme; RNA nanostructure; RNA nanotechnology; RNA-protein complex; trans-splicing

Funding

  1. University of Toyama Discretionary Funds of the President Toyama RNA Collaborative Research

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Ribozymes are attractive platforms for constructing nanoscale objects with biological functions. In this study, a dimeric form of the Tetrahymena group I ribozyme was designed and analyzed biochemically and observed directly by atomic force microscopy. The formation of the ribozyme dimer triggered trans-splicing reactions, resulting in a fluorescent RNA aptamer as the trans-splicing product.
Ribozymes are catalytic RNAs that are attractive platforms for the construction of nanoscale objects with biological functions. We designed a dimeric form of the Tetrahymena group I ribozyme as a unit structure in which two ribozymes were connected in a tail-to-tail manner with a linker element. We introduced a kink-turn motif as a bent linker element of the ribozyme dimer to design a closed trimer with a triangular shape. The oligomeric states of the resulting ribozyme dimers (kUrds) were analyzed biochemically and observed directly by atomic force microscopy (AFM). Formation of kUrd oligomers also triggered trans-splicing reactions, which could be monitored with a reporter system to yield a fluorescent RNA aptamer as the trans-splicing product.

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