Journal
ADVANCED SCIENCE
Volume 8, Issue 13, Pages -Publisher
WILEY
DOI: 10.1002/advs.202100962
Keywords
electrical stimulation; macrophage polarization; piezoelectric materials; proinflammatory; ultrasound
Categories
Funding
- National Key R&D Program of China [2017YFB0405400]
- National Natural Science Foundation of China [51732007]
- Natural Science Foundation of Shandong Province [ZR2020YQ35]
- Major Innovation Projects in Shandong Province [2018YFJH0503]
- Fundamental Research Funds of Shandong University [2018JC005]
- Project of 20 items of University of Jinan [2018GXRC031]
- Young Elite Scientist Sponsorship Program by CAST (YESS)
Ask authors/readers for more resources
The study proposes a method for regulating macrophage polarization using electrical signals derived from a piezoelectric material, which enhances the M1 polarization of macrophages, inhibits tumor cell proliferation, and provides a powerful tool for engineering macrophages for immunotherapy.
Proinflammatory (M1) macrophages play a vital role in antitumor immunity, and regulation of proinflammatory macrophage polarization is critical for immunotherapy. The polarization of macrophages can be regulated by biological or chemical stimulation, but investigations of the regulatory effect of physical stimulation are limited. Herein, regulating macrophage polarization with localized electrical signals derived from a piezoelectric beta-phase poly(vinylidene fluoride) (beta-PVDF) film in a wireless mode is proposed. Charges released on the surface of the beta-PVDF film driven by ultrasonic irradiation can significantly enhance the M1 polarization of macrophages. Mechanistic investigation confirms that electrical potentials rather than reactive oxygen species and mechanical forces enable Ca2+ influx through voltage-gated channels and establishment of the Ca2+-CAMK2A-NF-kappa B axis to promote the proinflammatory macrophage response during ultrasound treatment. Piezoelectric material-mediated electrical signal-activated proinflammatory macrophages significantly inhibit tumor cell proliferation. A method for electrogenetic regulation of immune cells as well as a powerful tool for engineering macrophages for immunotherapy is provided here.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available