Journal
NANOMATERIALS
Volume 11, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/nano11030768
Keywords
exosomes; quantitative detection; lateral flow assay; multi-quantum dots-embedded silica-encapsulated silica nanoparticle; test strip
Categories
Funding
- Seoul National University Research Grant in 2018
- National Research Foundation of Korea (NRF) [NRF2019M2D2A1A01058210, NRF-2020R1C1C1009000]
- Konkuk University [2017-A019-0334, 2020-A019-0280]
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A new technique utilizing highly bright multi-quantum dots embedded nanoparticles was introduced to improve exosome detection. This method achieved highly sensitive and quantitative detection of exosomes through lateral flow immunoassay.
Exosomes are attracting attention as new biomarkers for monitoring the diagnosis and prognosis of certain diseases. Colorimetric-based lateral-flow assays have been previously used to detect exosomes, but these have the disadvantage of a high limit of detection. Here, we introduce a new technique to improve exosome detection. In our approach, highly bright multi-quantum dots embedded in silica-encapsulated nanoparticles (M-QD-SNs), which have uniform size and are brighter than single quantum dots, were applied to the lateral flow immunoassay method to sensitively detect exosomes. Anti-CD63 antibodies were introduced on the surface of the M-QD-SNs, and a lateral flow immunoassay with the M-QD-SNs was conducted to detect human foreskin fibroblast (HFF) exosomes. Exosome samples included a wide range of concentrations from 100 to 1000 exosomes/mu L, and the detection limit of our newly designed system was 117.94 exosome/mu L, which was 11 times lower than the previously reported limits. Additionally, exosomes were selectively detected relative to the negative controls, liposomes, and newborn calf serum, confirming that this method prevented non-specific binding. Thus, our study demonstrates that highly sensitive and quantitative exosome detection can be conducted quickly and accurately by using lateral immunochromatographic analysis with M-QD-SNs.
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