Journal
MICROBIOLOGYOPEN
Volume 10, Issue 2, Pages -Publisher
WILEY
DOI: 10.1002/mbo3.1176
Keywords
chronological lifespan; Ecl1 family gene; fission yeast; GAAC; magnesium
Categories
Funding
- Japan Society for the Promotion of Science [JP16K07662, JP17H03792, JP17K19227, JP19K15730, JP20H02898]
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This study demonstrates that magnesium depletion extends the lifespan of yeast through the Ecl1 gene family and the general amino acid control pathway. The Ecl1 family genes regulate ribosome amount and contribute to longevity by activating the evolutionarily conserved GAAC pathway.
Nutrients including glucose, nitrogen, sulfur, zinc, and iron are involved in the regulation of chronological lifespan (CLS) of yeast, which serves as a model of the lifespan of differentiated cells of higher organisms. Herein, we show that magnesium (Mg2+) depletion extends CLS of the fission yeast Schizosaccharomyces pombe through a mechanism involving the Ecl1 gene family. We discovered that ecl1(+) expression, which extends CLS, responds to Mg2+ depletion. Therefore, we investigated the underlying intracellular responses. In amino acid auxotrophic strains, Mg2+ depletion robustly induces ecl1(+) expression through the activation of the general amino acid control (GAAC) pathway-the equivalent of the amino acid response of mammals. Polysome analysis indicated that the expression of Ecl1 family genes was required for regulating ribosome amount when cells were starved, suggesting that Ecl1 family gene products control the abundance of ribosomes, which contributes to longevity through the activation of the evolutionarily conserved GAAC pathway. The present study extends our understanding of the cellular response to Mg2+ depletion and its influence on the mechanism controlling longevity.
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